Search results for "Fusarium langsethiae"

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Different sample treatment approaches for the analysis of T-2 and HT-2 toxins from oats-based media.

2010

A LC-DAD method is proposed for the determination of the T-2 and HT-2 toxins in cultures of Fusarium langsethiae in oat-based and other in vitro media. Test media consisted of freshly prepared milled oats to which T-2 and HT-2 toxin stock solutions were added. Different mixtures of extraction solvent (acetonitrile:water and methanol water), extraction times (30', 60' or 90') and drying methods were investigated. Results showed that extraction with methanol: water (80:20, v/v) for 90 min, drying with N-2 and subsequent analysis by LC-DAD was the fastest and most user friendly method for detecting HT-2 and T-2 toxins production by F. langsethiae strains grown on oat-based media at levels of 0…

food.ingredientTime FactorsWater activityAvenaClinical BiochemistryTrichotheceneBiochemistryAnalytical Chemistrychemistry.chemical_compoundfoodFusariumAnalysis Type A trichothecenes Diode array Cereals performance liquid-chromatography diode-array detection gas-chromatography mass-spectrometry immunoaffinity cleanup fluorescence detection fusarium-langsethiae retention indexes b-trichothecene cerealsGlycerolAgarSample preparationDesiccationChromatographybiologyAnalytic Sample Preparation MethodsCell BiologyGeneral MedicineReference Standardsbiology.organism_classificationCulture MediaFusarium langsethiaeT-2 ToxinAvenachemistrySolventsMethanolChromatography LiquidJournal of chromatography. B, Analytical technologies in the biomedical and life sciences
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